A Comparison of Two Separation Modes: HILIC and Aqueous Normal Phase Chromatography
نویسندگان
چکیده
In the literature (1) and in commercial advertising, one can often find material descriptions of chromatographic retention that use the terms hydrophilic interaction liquid chromatography (HILIC) and aqueous normal phase (ANP) in such a manner that they can be construed to be identical. However, in reality, there should be (and there is) a real difference between these two modes of retention and the types of columns that achieve them. A more precise definition of ANP and HILIC is proposed here that will enable chromatographers to distinguish between the two mechanisms and select columns suitable for particular applications.
منابع مشابه
Hydrophilic interaction chromatography
In the early 1990s, hydrophilic interaction (liquid) chromatography (HILIC) emerged as a new separation method. HILIC is becoming increasingly popular for the separation of polar compounds in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). It has become important for the separation of pharmaceuticals, neurotransmitters, nucleosides, nucleotides and other compounds...
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